Dear Cell Cyclists,
Summer has hit Sweden, so it’s actually not the right time to spend locked-up in the lab doing microscopy. However, I collated 4 nice resources around the topic high-content fluorescent microscopy covering the whole range from basic concepts in microscopy to advanced image analysis.
1. A video crash course to microscopy and digital images. Introducing concepts as brightness/contrast, bit-depth, gamma, LUTs, compression, file formats…
2. A very nice paper introducing quantitative analysis of fluorescence microscopy images for cell-based screening
3. A great introduction to microscopy-based high-content screening
4. And finally a paper describing how to automatically identify image acquisition artifacts such as dirt or out-of-focus images (providing also a CellProfiler pipeline)